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Journal: Molecular Medicine
Article Title: Alpha-1 antitrypsin–glucocorticoid receptor axis: a new pathway in immune modulation
doi: 10.1186/s10020-026-01470-z
Figure Lengend Snippet: Endogenous AAT and GR co-localize in human PBMCs. Co-staining of adherent human blood PBMCs with anti-AAT ( red ) and polyclonal ( A ) or monoclonal ( B ) anti-GR ( green ) antibodies reveals co-localization of AAT and GR in the cytoplasm. Nuclei were stained with DAPI. Images were taken using a confocal laser scanning microscope (Olympus FluorView 1000) equipped with a 60× oil-immersion objective. Scale bars represent 20 μm
Article Snippet: Adherent cells were fixed with 4% paraformaldehyde (PFA) for 10 min at 37 °C, permeabilized with 0.05% Triton-X100 for 5 min and blocked with 5% BSA in PBS for 1 h. AAT was stained with monoclonal mouse-anti AAT antibody (Proteintech, dilution 1:200) and GR was stained with polyclonal rabbit anti-GR antibody (Thermo Fisher Scientific, dilution 1:100) or
Techniques: Staining, Laser-Scanning Microscopy